Other

Part:BBa_K2230004:Design

Designed by: Yi-Lun Huang   Group: iGEM17_Mingdao   (2017-10-19)


RBS-EmR-CP29-RBS-aeBlue/pLBA169


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4909
    Illegal suffix found in sequence at 1554
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4909
    Illegal SpeI site found at 1555
    Illegal PstI site found at 1569
    Illegal NotI site found at 1562
    Illegal NotI site found at 4915
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4909
    Illegal XhoI site found at 3195
    Illegal XhoI site found at 4087
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4909
    Illegal suffix found in sequence at 1555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4909
    Illegal XbaI site found at 4924
    Illegal SpeI site found at 1555
    Illegal PstI site found at 1569
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No


Source

RBS-EmR which was cut and gel-eluted from RBS-EmR/pSB1C3 (BBa_K2230002) was assembled with CP29-RBS-aeBlue/pLBA169 (BBa_K2230000)


References